A Word About

Liquid Chromatography

Chromatogram representing a typical ion exchange chromatography process (by GE). The blue line represents the continuous measurement of UV light absorbance of the liquid flowing out of the chromatography column.

Liquid chromatography is one of several general types of chromatography and is the type used most in biopharmaceutical production. In liquid chromatography, liquid is pumped through a container called a chromatography column. The column is packed tightly with a medium, usually tiny synthetic beads known as "gel", an arrangement that bears some resemblance in concept to a sand filter on a swimming pool. Materials dissolved in the liquid are separated based on relative differences in how fast they move through the medium.

Mechanically, liquid chromatography can be performed using several media formats, including:

Large-scale liquid chromatography can be performed in several modes, including:


Page updated 7/6/17


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